Description
Description: This assay employs a two-site sandwich ELISA to quantitate GNAT1 in samples. An antibody specific for GNAT1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any GNAT1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for GNAT1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of GNAT1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Overview: Transducin is a 3-subunit guanine nucleotide-binding protein (G protein) which stimulates the coupling of rhodopsin and cGMP-phoshodiesterase during visual impulses. The transducin alpha subunits in rods and cones are encoded by separate genes. GNAT1 encodes the alpha subunit in rods. Alternative splicing of this gene results in two transcript variants. The deduced 350-amino acid transducin protein, which is 100% identical to the mouse protein, contains a G-alpha domain and an ARF domain. Northern blot analysis revealed abundant expression of a 1.5-kb transcript in retina and fetal heart and in T-cell lines. No expression was detected in lung or lung cancer cell lines, and no mutations were found in any lung cancer cell lines